C3DNA — COVALENTLY CLOSED AND CIRCULAR DNA – IS INTERGALACTIC’S VERSATILE NON-VIRAL CARGO PLATFORM.
C3DNA allows for design and delivery of DNA vectors beyond the size limits of viral delivery systems.
Intergalactic precisely engineers C3DNA, using synthetic biology tools to assemble modular elements into a closed loop. This covalently closed, circular (C3) construction allows C3DNA to be taken up by cells and expressed, without insertion into the host genome.
Moreover, C3DNA avoids triggering an immune response, because it does not contain unintended viral or bacterial sequences. Therefore, the immune system does not “see” it, enabling C3DNA to act like an endogenous gene in the nucleus, with enhanced persistence of expression.
Our lead delivery modality for C3DNA therapies is COMET: Cellular delivery of genetic Material by Electro-Transfer. COMET, Intergalactic’s proprietary pulsed electric field local gene therapy delivery system, combines novel electrode design, custom wavelengths and algorithms, and electrical field modeling in a simple yet sophisticated device design. COMET devices and settings are precisely engineered to work with different types of C3DNA therapeutic payloads and to target specific tissue.
